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KMID : 0191120120270080857
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Journal of Korean Medical Science
2012 Volume.27 No. 8 p.857 ~ p.863
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Isolation and Characterization of Chorionic Mesenchymal Stromal Cells from Human Full Term Placenta
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Koo Bo-Kyung
Park In-Yang
Kim Ji-Yeon
Kim Ji-Hyun
Kwon Ahlm
Kim Myung-Shin
Kim Yong-Goo
Shin Jong-Chul
Kim Jong-Hoon
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Abstract
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This study focused on the characterization of mesenchymal stromal cells (MSCs) from the chorion of human full term placenta from 15 donors. Chorionic MSCs revealed homologous fibroblast-like morphology and expressed CD73, CD29, CD105, and CD90. The hematopoietic stem cell markers including HLA DR, CD11b, CD34, CD79a, and CD45 were not expressed. The growth kinetics of their serial passage was steady at the later passages (passage 10). The multilineage capability of chorionic MSCs was demonstrated by successful adipogenic, osteogenic and chondrogenic differentiation and associated gene expression. Chorionic MSCs expressed genes associated with undifferentiated cells (NANOG, OCT4, REX1) and cardiogenic or neurogenic markers such as SOX2, FGF4, NES, MAP2, and NF. TERT was negative in all the samples. These findings suggest that chorionic MSCs undifferentiated stem cells and less likely to be transformed into cancer cells. A low HLA DR expression suggests that chorionic MSCs may serve as a great source of stem cells for transplantation because of their immune-privileged status and their immunosuppressive effect. Based on these unique properties, it is concluded that chorionic MSCs are pluripotent stem cells that are probably less differentiated than BM-MSCs, and they have considerable potential for use in cell-based therapies.
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KEYWORD
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Chorion, Mesenchymal Stromal Cells, Human Placenta, Gene Expression
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